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Image Search Results
Journal: iScience
Article Title: Elimination of cells with local mismatch in differentiation timing contributes to synchronize tissue development
doi: 10.1016/j.isci.2025.113135
Figure Lengend Snippet: Heterochrony promotes cell elimination of both properly timed and heterochronous cells (A) Confocal images of control, Svb REP or Svb ACT clones stained with GFP (blue, clonal cells) and Dcp1 (yellow, apoptotic cells). White arrowheads point to some apoptotic cells within the clone, while empty arrowheads point to some non-clonal apoptotic cells that are in direct contact with clonal cells. Scale bar: 10 μm. (B) Graph showing the percentage of clones (GFP+ cells) containing at least one apoptotic cell (Dcp1+ cell) in the three conditions. Clones were analyzed on 16/14/15 nota in control, Svb REP and Svb ACT conditions, with 822, 485, and 453 clonal cells, respectively. (C) Graph showing the density of clones (number of clones normalized per mm 2 ), in the same samples as (B). (D) Graph showing normalized number of Dcp1 positive cells in control, Svb REP or Svb ACT conditions, according to their distance from the clone: clonal cells (clone), cells which are direct neighbors (i.e., at the clone contact, row +1) or cells which are separated from the nearest clone by one (row +2), two (row +3) or more cell diameters (row >3). Data are from 7 nota with 231 clonal cells and 321 Dcp1+ cells in controls, 7 nota with 1536 clonal and 463 Dcp1+ cells in Svb REP , 7 nota with 324 clonal and 306 Dcp1+ cells in Svb ACT conditions. In (B), (C), and (D), data are represented as mean +/− standard deviations and individual values are shown in (B) and (C). Statistics: Mann-Whitney tests (B) or t tests (C and D). p value: <0.05, ∗; p < 0.01, ∗∗; p < 0.0001, ∗∗∗∗; ns (non-significant) >0.05.
Article Snippet:
Techniques: Control, Clone Assay, Staining, MANN-WHITNEY